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Immunohistochemical expression of <t>CHI3L1.</t> (A) Scoring system for CHI3L1 staining in glioblastoma. (B) Example of CHI3L1 reactivity in gliosarcoma, with labeling of glial (GFAP-high reticulin-low) but not sarcomatous (GFAP-low reticulin-high) elements. (C) Proportions of glioblastoma (left bar) or glial regions of gliosarcoma (right bar) staining at each intensity level. (D) Patterns of CHI3L1 staining in gliosarcomas; see text for details. Scale bars 50 μm (A, D) and 200 μm (B).
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Average 95 stars, based on 1 article reviews
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rabbit anti chi3l1  (Proteintech)
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Proteintech rabbit anti chi3l1
Immunohistochemical expression of <t>CHI3L1.</t> (A) Scoring system for CHI3L1 staining in glioblastoma. (B) Example of CHI3L1 reactivity in gliosarcoma, with labeling of glial (GFAP-high reticulin-low) but not sarcomatous (GFAP-low reticulin-high) elements. (C) Proportions of glioblastoma (left bar) or glial regions of gliosarcoma (right bar) staining at each intensity level. (D) Patterns of CHI3L1 staining in gliosarcomas; see text for details. Scale bars 50 μm (A, D) and 200 μm (B).
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Immunohistochemical expression of CHI3L1. (A) Scoring system for CHI3L1 staining in glioblastoma. (B) Example of CHI3L1 reactivity in gliosarcoma, with labeling of glial (GFAP-high reticulin-low) but not sarcomatous (GFAP-low reticulin-high) elements. (C) Proportions of glioblastoma (left bar) or glial regions of gliosarcoma (right bar) staining at each intensity level. (D) Patterns of CHI3L1 staining in gliosarcomas; see text for details. Scale bars 50 μm (A, D) and 200 μm (B).

Journal: bioRxiv

Article Title: Clinical and molecular features of primary gliosarcoma with digital spatial whole-transcriptome analysis of glial and mesenchymal components

doi: 10.1101/2025.09.02.673845

Figure Lengend Snippet: Immunohistochemical expression of CHI3L1. (A) Scoring system for CHI3L1 staining in glioblastoma. (B) Example of CHI3L1 reactivity in gliosarcoma, with labeling of glial (GFAP-high reticulin-low) but not sarcomatous (GFAP-low reticulin-high) elements. (C) Proportions of glioblastoma (left bar) or glial regions of gliosarcoma (right bar) staining at each intensity level. (D) Patterns of CHI3L1 staining in gliosarcomas; see text for details. Scale bars 50 μm (A, D) and 200 μm (B).

Article Snippet: Immunohistochemistry for CHI3L1 was performed using CHI3L1 antibody (Proteintech 12036-1-AP), antigen retrieval with citrate buffer pH 6.0 at 80° C for 45 minutes, blocking by 3% milk solution for 15 minutes, and primary antibody 1:500 overnight at 4° C. Secondary antibody (Vector Laboratories BA-1000 1:800) was applied for 1 hour at room temperature and detected by incubation with DAB solution.

Techniques: Immunohistochemical staining, Expressing, Staining, Labeling